Neutrophils Induced Licensing of Natural Killer Cells
Neutrophils promote a licensing effect of NK cells: CD107a degranulation assay. Forty Gy-irradiated 2 × 106 mononuclear cells (splenocyte, SPL; peripheral blood, PB; and G-CSF-treated PB, G-PB) of B10.D2 mice were injected intraperitoneally into B10 mice. Two days after injection of cells, peripheral blood mononuclear cells of recipient B10 mice were analyzed by flow cytometry. Representative histograms show CD107a expressions gated on DX5+CD3−Ly49G2+ cells ((a), left) and DX5+CD3−Ly49C/I+ cells ((a), right) in treated B10 mice (solid line) and untreated as controls (shadow area). Time course of mean fluorescence intensity (MFI) of CD107a expressions of DX5+CD3−Ly49G2+ cells ((b), left) and DX5+CD3−Ly49C/I+ cells ((b), right) was analyzed before () and after injection of SPL (open circle, ), PB (open triangle, ), and G-PB (filled square, ). Data are expressed as the means ± SD. ; .
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