Neutrophils Induced Licensing of Natural Killer Cells

Figure 3

Neutrophils promote a licensing effect of NK cells: upregulation of intracellular IFN-γ expression. Forty Gy-irradiated 2 × 10⁶ mononuclear cells (PB and G-PB) of B10.D2 mice were injected intraperitoneally into B10 mice. Two days after injection, PBMCs of recipient B10 mice were analyzed by flow cytometry. Representative dot plots show intracellular IFN-γ expressions gated on DX5⁺CD3⁻Ly49G2⁺ NK cells ((a), upper panel) and DX5⁺CD3⁻Ly49C/I⁺ NK cells ((a), lower panel) in uninjected B10 PBMCs (control), B10 PBMCs injected by B10.D2 PBMCs (PB), and B10 PBMCs injected by G-CSF-treated B10.D2 PBMCs (G-PB). The comparison of IFN-γ expression of DX5⁺CD3⁻Ly49G2⁺ NK cells and DX5⁺CD3⁻Ly49C/I⁺ NK cells in control (), PB (), and G-PB () was depicted (b). Data are expressed as the means ± SD. ; .

(a)

(b)