Neutrophils Induced Licensing of Natural Killer Cells
Neutrophils promote a licensing effect of NK cells: upregulation of intracellular IFN-γ expression. Forty Gy-irradiated 2 × 106 mononuclear cells (PB and G-PB) of B10.D2 mice were injected intraperitoneally into B10 mice. Two days after injection, PBMCs of recipient B10 mice were analyzed by flow cytometry. Representative dot plots show intracellular IFN-γ expressions gated on DX5+CD3−Ly49G2+ NK cells ((a), upper panel) and DX5+CD3−Ly49C/I+ NK cells ((a), lower panel) in uninjected B10 PBMCs (control), B10 PBMCs injected by B10.D2 PBMCs (PB), and B10 PBMCs injected by G-CSF-treated B10.D2 PBMCs (G-PB). The comparison of IFN-γ expression of DX5+CD3−Ly49G2+ NK cells and DX5+CD3−Ly49C/I+ NK cells in control (), PB (), and G-PB () was depicted (b). Data are expressed as the means ± SD. ; .
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