Neutrophils Induced Licensing of Natural Killer Cells

Figure 4

Enrichment and depletion of neutrophils affect licensing effect of NK cells. B10.D2 PB was enriched or depleted of CD11b⁺Gr-1⁺ neutrophils by magnet beads negative selection (a). Forty Gy-irradiated 2 × 10⁶ PBMCs or neutrophil-enriched/depleted cells were injected intraperitoneally into B10 mice. Two days after injection, PBMCs of recipient B10 mice were analyzed by flow cytometry. Representative dot plot analysis showed intracellular IFN-γ expressions gated on DX5⁺CD3⁻Ly49G2⁺ NK cells ((b), upper panel) and DX5⁺CD3⁻Ly49C/I⁺ NK cells ((b), lower panel) in uninjected B10 PBMCs (control), B10 PBMCs injected by B10.D2 PBMCs (PB untreated), B10.D2 PBMCs enriched neutrophils (neutrophil rich), and B10.D2 PBMCs depleted of neutrophils (neutrophil depleted). The comparison of IFN-γ expression of DX5⁺CD3⁻Ly49G2⁺ NK cells and DX5⁺CD3⁻Ly49C/I⁺ NK cells in control (), PB untreated (), neutrophil rich (), and neutrophil depleted () was depicted (c). Data are expressed as the means ± SD. ; .

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