Erythropoietin Exacerbates Inflammation and Increases the Mortality of Histoplasma capsulatum-Infected Mice

Figure 4

EPO pretreatment alters the expression of macrophage activation markers in Hc-infected mice. 14 days after infection, BALF cell populations from uninfected mice EPO-treated (EPO) and from untreated (Hc) or EPO pretreated (EPO + Hc) mice infected with Hc (5 × 10⁵ yeasts/100 μL/mouse) were analyzed by flow cytometry for the percentage of F4/80⁺CD80⁺ (a), F4/80⁺CD86⁺ (b), and F4/80⁺MHC II⁺ (c) cells. Means ± SEM values from one representative of two independent experiments are shown (–6). Newman-Keuls multiple comparison test was used and the differences were considered significant when . ^#Versus Hc; uninfected and EPO-untreated (dashed line); ^&versus uninfected and EPO-treated mice.

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