Erythropoietin Exacerbates Inflammation and Increases the Mortality of Histoplasma capsulatum-Infected Mice

Figure 6

EPO pretreatment alters PGE₂ and LTB₄ production in lungs from Hc-infected mice. Lungs were removed from uninfected mice treated with 45 U EPO (EPO) or from untreated (Hc) or EPO pretreated (EPO + Hc) mice infected with Hc (5 × 10⁵ yeasts/100 μL/mouse). Lungs were removed 14 days after infection and homogenized, and eicosanoids were purified as described in the Materials and Methods. PGE₂ (a) and LTB₄ (b) concentration was quantified in the purified homogenates by HPLC-MS/MS. Means ± SEM values from one representative experiment are shown (–6). Newman-Keuls multiple comparison test was used and the differences were considered significant when . ^#Versus Hc; uninfected and EPO-untreated (dashed line); ^&versus uninfected and EPO-treated mice.

(a)

(b)