Atorvastatin Improves Inflammatory Response in Atherosclerosis by Upregulating the Expression of GARP

Figure 1

Atorvastatin upregulated the expression of GARP in anti-CD3/CD28 stimulated splenic CD4+ T cells: CD4+ T cells were isolated by magnetic activated cell sorting (MACS). (a) The relative mRNA expression of GARP in atorvastatin cocultured stimulated splenic CD4+ T cells, in vitro. Repeated times , and compared with the control wells. (b) The relative protein expression of GARP in atorvastatin cocultured stimulated splenic CD4+ T cells, in vitro. Repeated times , and compared with the control wells. (c) The relative mRNA expression of GARP in stimulated splenic CD4+ T cells derived from mice in scrambled group (scrambled), oral atorvastatin + scrambled group (O.A. + scrambled), and oral atorvastatin + GARP-siRNA group (O.A. + GARP-siRNA), in vivo. Data were from 6 mice per group () and were independently confirmed, all , , and . (d) The relative protein expression of GARP in stimulated splenic CD4+ T cells derived from mice in these three groups, in vivo. Data were from 6 mice per group () and were independently confirmed, all , , and . (e) The relative mRNA expression of TGF-β in stimulated splenic CD4+ T cells derived from mice in these three groups, in vivo. Data were from 6 mice per group () and were independently confirmed, all , , and . (f) The protein expression of TGF-β in stimulated splenic CD4+ T cells derived from these three groups, in vivo. Data were from 6 mice per group () and were independently confirmed, all , , and . (g) The relative protein expression of total Smad3 and phosphorylated Smad3 (P-Samd3) in stimulated splenic CD4+ T cells derived from mice in these three groups, in vivo. Data were from 6 mice per group () and were independently confirmed, all and .

(a)

(b)

(c)

(d)

(e)

(f)

(g)