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Mediators of Inflammation
Volume 2015 (2015), Article ID 879126, 6 pages
Clinical Study

Sensory Neuropeptides and Endogenous Opioids Expression in Human Dental Pulp with Asymptomatic Inflammation: In Vivo Study

1Faculty of Dentistry, Costa Rica University, 1493-3000 San José, Costa Rica
2Endodontics Postgraduate Program, Faculty of Dentistry, San Luis Potosi University, 78290 San Luis Potosi, SLP, Mexico
3Basic Sciences Laboratory, Faculty of Dentistry, San Luis Potosi University, 78290 San Luis Potosi, SLP, Mexico
4Research and Development, Axopod Consultants for Life, 110921 Bogotá, Colombia

Received 29 May 2015; Revised 1 July 2015; Accepted 7 July 2015

Academic Editor: Sang Hyuk Park

Copyright © 2015 Daniel Chavarria-Bolaños et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Purpose. This study quantified the expression of substance P (SP), calcitonin gene-related peptide (CGRP), β-endorphins (β-End), and methionine-enkephalin (Met-Enk) in human dental pulp following orthodontic intrusion. Methods. Eight patients were selected according to preestablished inclusion criteria. From each patient, two premolars (indicated for extraction due to orthodontic reasons) were randomly assigned to two different groups: the asymptomatic inflammation group (EXPg), which would undergo controlled intrusive force for seven days, and the control group (CTRg), which was used to determine the basal levels of each substance. Once extracted, dental pulp tissue was prepared to determine the expression levels of both neuropeptides and endogenous opioids by radioimmunoassay (RIA). Results. All samples from the CTRg exhibited basal levels of both neuropeptides and endogenous opioids. By day seven, all patients were asymptomatic, even when all orthodontic-intrusive devices were still active. In the EXPg, the SP and CGRP exhibited statistically significant different levels. Although none of the endogenous opioids showed statistically significant differences, they all expressed increasing trends in the EXPg. Conclusions. SP and CGRP were identified in dental pulp after seven days of controlled orthodontic intrusion movement, even in the absence of pain.