Blockage of Eosinopoiesis by IL-17A Is Prevented by Cytokine and Lipid Mediators of Allergic Inflammation

Figure 1

Effect of IL-17A on eosinophil production from murine bone-marrow. Bone-marrow cultures were established from BALB/c mice in the presence of IL-5 (1 ng/mL), alone or in association with the indicated concentrations of IL-17A. After the indicated periods, cells were collected, counted, and stained for eosinophil peroxidase (EPO). Total and EPO+ cell counts were performed, and data (mean ± SEM, ) are the numbers of EPO+ cells present at the end of the culture. (a) Effect of varying IL-17A concentration on EPO+ cell numbers at day 7. Insert: effect of culture in the absence of IL-5; open and closed bars: cultures established, respectively, with and without IL-17A. (b) Effect of increasing duration of culture. (c) Effect of variable timing of IL-17A addition relative to the initiation of the culture. (d) Effect of blocking terminal caspases with zVAD-fmk (z-VAD) (20 μM). (e) Annexin V-FITC staining of cells in the granulocyte gate recovered from BALB/c and GATA-1 day 5 bone-marrow cultures. Data are mean ± SEM () of the % granulocytes in the gated region (FL-1 positive, FL-2 negative). At day 2, there was no significant difference between IL-17A-exposed and control cultures from BALB/c mice (not shown). for the differences between the indicated points and the respective IL-5 (negative) controls.

(a)

(b)

(c)

(d)

(e)