Inflammation Biomarkers of Advanced Disease in Nongingival Tissues of Chronic Periodontitis Patients

Figure 2

Cytokine expression in alveolar bone tissue of control and periodontal disease (PD) patients. Total RNA extracted from bone biopsies of PD and control group was subjected to qRT-PCR, SYBR green method. RNA expression of TNF-α (a), IFN-γ (b), IL-10 (c), and IL-4 (d) is presented as the intensity of expression of the individual mRNAs, with normalization to β-actin, using the cycle threshold method. Data are the mean ± SEM results for patients with PD and control subjects tested individually. The results shown are from one experiment representative of three. .

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