Inflammation Biomarkers of Advanced Disease in Nongingival Tissues of Chronic Periodontitis Patients

Table 2

Human primers sequences for real-time PCR used in this study.

Genes	Sequence (5′→3′)
-actin	S - TGA CTC AGG ATT TAA AAA CTG GAA
	AS - GCC ACA TTG TGA ACT TTG GG
TNF-	S - TTC TGG CTC AAA AAG AGA ATT G
	AS - TGG TGG TCT TGT TGC TTA AAG
IFN-	S - GAG AAC CCA AAA CGA TGC A
	AS - ACT TCT TTG GCT TAA TTC TCT CG
IL-4	S - GAA GGA AGC CAA CCA GAG TA
	AS - GAT CGT CTT TAG CCT TTC CA
IL-10	S - TTC CCT GAC CTC CCT CTA ATT
	AS - GCT CCC TGG TTT CTC TTC CTA A
MMP-1	S - GAT TGA AAA TTA CAC GCC AGA T
	AS - TCT CAA TGG CAT GGT CCA
MMP-2	S - ACT GTT GGT GGG AAC TCA GA
	AS - TTG TTG CCC AGG AAA GTG
MMP-3	S - AAG GAT ACA ACA GGG ACC AA
	AS - CAG TGT TGG CTG AGT GAA AG
MMP-9	S - TCA CTT TCC TGG GTA AGG AGT A
	AS - TGT CAA AGT TCG AGG TGG TAG

S: sense; AS: antisense.