PGE2 Elevates IL-23 Production in Human Dendritic Cells via a cAMP Dependent Pathway

Figure 1

Influence of PGE2 on IL-23 expression in human Mo-DCs and human monocytes. (a, c, and d) Mo-DCs or (b) human monocytes were pretreated for 4 h with PGE2 (0–10 μM), PGE2 (2 μM), or solvent control DMSO. Then the cells were incubated with zymosan (20 μg/mL) in the presence or absence of PGE2 for 24 h (ELISA) or 5 h (RT-PCR). Expression levels of IL-23 protein were determined by ELISA 24 h after stimulation. Expression of (c) IL-23 p19 and (d) IL-23 p40 was analyzed by RT-PCR 5 hours after stimulation. Data represent mean ± SEM of 5 separate experiments. versus zymosan alone; versus zymosan alone.

(a)

(b)

(c)

(d)