Krüppel-Like Factor 4 Is a Regulator of Proinflammatory Signaling in Fibroblast-Like Synoviocytes through Increased IL-6 Expression

Figure 1

KLF4 expression in synovial tissue and FLSs from RA patients. Real-time PCR (a) and western blot (b) were performed to assess KLF4 expression in synovial tissues from patients with RA () and OA (). GAPDH as an endogenous control. in comparison to OA group. (c) KLF4 expression in synovial tissues from patients with RA was assessed by immunohistochemistry. Arrows indicate KLF4-positive cells (200x magnification). Real-time PCR (d) and western blot (e) were performed to assess KLF4 expression in isolated FLSs from patients with RA () and OA (). GAPDH as an endogenous control. in comparison to OA group. (f) KLF4 expression in isolated FLSs from patients with RA was assessed by immunofluorescence. Cell nuclei were visualized by Hoechst 33342 staining (100x magnification).

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