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Mediators of Inflammation
Volume 2016, Article ID 1340156, 11 pages
http://dx.doi.org/10.1155/2016/1340156
Research Article

Bidirectional Crosstalk between C5a Receptors and the NLRP3 Inflammasome in Macrophages and Monocytes

Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109, USA

Received 24 February 2016; Revised 4 May 2016; Accepted 15 May 2016

Academic Editor: Magdalena Klink

Copyright © 2016 Mikel D. Haggadone et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The supplementary materials include three figures. In Supplementary Figure 1, western blot results are presented to demonstrate detection of processed, but not unprocessed, IL-1β in our macrophage and bone marrow cell NLRP3 inflammasome stimulation experiments. Furthermore, these data corroborate the ELISA results presented throughout our manuscript by showing alterations in myeloid cell IL-1β production evoked by C5a. Supplementary Figure 2 shows that C5a only modestly regulates NLRP3 inflammasome activity in peritoneal neutrophils. Supplementary Figure 3 presents the data shown in Figure 4 as absolute amounts of IL-1β protein released as opposed to the percent of IL-1β protein released relative to positive control samples.

  1. Supplementary Material