Epobis is a Nonerythropoietic and Neuroprotective Agonist of the Erythropoietin Receptor with Anti-Inflammatory and Memory Enhancing Effects

Figure 5

Effects of Epobis on the clinical signs of EAE. Following induction of EAE the clinical signs were evaluated daily for all animals. Only animals reaching a clinical score ≥1 before day 14 were included in the study. EAE was induced in 30 animals of which 20 developed clinical signs of EAE (10 receiving PBS; 10 receiving Epobis). In the figure, the data have been aligned according to the onset of clinical signs that were scored as follows: 0, no abnormality; 0.5, weak tail; 1, limp tail; 2, mild palsy of one or both hind legs; 3, severe palsy of one or both hind legs; 4, complete paralysis of one or both hind legs; 5, paralysis of one or both hind legs and beginning paralysis of front legs; 6, moribund. Animals with a clinical score ≥4 were sacrificed immediately. The clinical scores of animals sacrificed due to a clinical score ≥4 have been maintained at all subsequent time points. Data are shown as mean and SEM. The data were evaluated using a 2-way ANOVA for repeated measures followed by a Bonferroni posttest. relative to the corresponding control animals.