Research Article

Forskolin Inhibits Lipopolysaccharide-Induced Modulation of MCP-1 and GPR120 in 3T3-L1 Adipocytes through an Inhibition of NFB

Figure 7

mRNA expression of MCP-1 (a), TLR-4 (b), GPR120 (c), and NFκB1 (d) in differentiated 3T3-L1 cells treated with LPS in the presence or absence of inhibitors. Differentiated 3T3-L1 cells were treated as described in Materials and Methods under the following conditions: CTL, 1 µg/mL LPS, 3 µM CLI-095 + 1 µg/mL LPS (CLI + LPS), and 10 µM of BAY11-7082 + 1 µg/mL LPS (BAY + LPS). The results are expressed as mRNA levels (fold stimulation over CTL set to 1) and are the means ± SEM of 3 independent experiments. Data were analyzed using -test for unique sample and paired -test; ; versus CTL; ; versus LPS.
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