Sphingosine-1-Phosphate/Sphingosine-1-Phosphate Receptor 2 Axis Can Promote Mouse and Human Primary Mast Cell Angiogenic Potential through Upregulation of Vascular Endothelial Growth Factor-A and Matrix Metalloproteinase-2

Figure 1

Role of S1P/S1PR2 signaling in VEGF-A secretion. (a) Blood was collected from allergenically challenged WT (open bar, -6 mice) and S1PR2-null (black bar, -6 mice) mice, euthanized 2 hours after Ag challenge, and serum VEGF-A levels were measured in duplicate determination for each animal. (b) Bone marrow-derived mast cells (BMMC, three independent populations) from both genotypes were stimulated for 24 hours with vehicle (DMSO/PBS/4 mg/mL fatty acid-free BSA), S1P (100 nM), IgE/Ag, or ionomycin (Iono) and VEGF-A was measured in MC supernatants, in duplicate determinations. Error bars show standard error of means. , .

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