FFAR4 (GPR120) Signaling Is Not Required for Anti-Inflammatory and Insulin-Sensitizing Effects of Omega-3 Fatty Acids

Table 2

Primer sequences and annealing temperatures. Primer sequences and annealing temperature are depicted for relevant primer pairs.

Primers	Primer (forward, reverse)	Annealing temp.
Acox1	5′GGGTCATGGAACTCATCTTCGA	58°C
	5′GAATGAACTCTTGGGTCTTGGG
Cd68	5′CTTCCCACAGGCAGCACAG	61°C
	5′AATGATGAGAGGCAGCAAGAGG
Fas	5′ATTGGTGGTGTGGACATGGTC	61°C
	5′CCCAGCCTTCCATCTCCTG
Il-6	5′CTCTGCAAGAGACTTCCATCCAGT	60°C
	5′GAAGTGGTATAGACAGGTCTGTTGG
Irs2	5′TCTGCCAGCACCTATGCAA	60°C
	5′GCTTCACTCTTTCACGACTGTG
Mcad	5′AGTATGCCCTGGATAGGAAGACAT	60°C
	5′CTTGGTGCTCCACTAGCAGCT
Mcp-1	5′GTGTTGGCTCAGCCAGATGC	62°C
	5′GCTTGGTGACAAAAACTACAGC
Nrf-1	5′CAGCACCTTTGGAGAATGTG	55°C
	5′CCTGGGTCATTTTGTCCACA
Pparγ2	5′ACAGCAAATCTCTGTTTTATGC	60°C
	5′TGCTGGAGAAATCAACTGTGG
Scd1	5′ACACCTGCCTCTTCGGGATT	61°C
	5′TGATGCCCAGAGCGCTG
Socs3	5′GCCTTTCAGTGCAGAGTAGTG	63°C
	5′AAGAGCAGGCGAGTGTAGAG
Tbp	5′ACCCTTCACCAATGACTCCTATG	60°C
	5′ATGATGACTGCAGCAAATCGC
Tnfα	5′CCCTCACACTCAGATCATCTTCT	63°C
	5′GCTACGACGTGGGCTACAG