CYLD Promotes TNF-α-Induced Cell Necrosis Mediated by RIP-1 in Human Lung Cancer Cells

Figure 3

Transfection of pcDNA3.1(+)-CYLD plasmid increases CYLD expression in lung cancer cell lines. (a) The lung cancer cells A549 and H460 were transfected with the recombinated plasmid pcDNA3(+)/CYLD-flag and control plasmid pcDNA3.1(+) for 24 hours. The levels of CYLD were detected by in lung cancer cell lines western blotting analysis. (b) The images were captured by image J software and gray values were analyzed and shown in histogram. , compared with control plasmid. (c) A549 cells were transfected with CYLD-flag plasmid and cultured for 24 h, 48 h, and 72 h, respectively. The cell viability was determined by MTT assay. , , compared with the control plasmid transfected cells. (d) H460 cells were transfected with CYLD-flag plasmid for 24 h, 48 h, and 72 h. The cell viability was determined by MTT assay. , , compared with the control plasmid transfected cells.

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