CD97/ADGRE5 Inhibits LPS Induced NF-κB Activation through PPAR-γ Upregulation in Macrophages

Figure 3

Effect of CD97 manipulations on p65 through increasing PPAR-γ expression. (a) Pathways analysis when macrophages were transfected with CD97 overexpression plasmids. (b) CD97 ablation inhibited PPAR-γ protein level while its ectopic expression increased it. (c) LPS inhibited PPAR-γ protein levels in a concentration dependent gradient (from 0 to 60 ng/mL) (A). CD97 ectopic expression rescued LPS induced PPAR-γ inhibition (40 ng/mL) (B). (d) CD97 induced p65 inhibition was rescued by PPAR-γ ablation by siRNA.

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