CD97/ADGRE5 Inhibits LPS Induced NF-κB Activation through PPAR-γ Upregulation in Macrophages

<div>Effect of CD97 knockout on PPAR-γ and p65 expression in THP-1 cells. (a) Validation of CD97 knockout in THP-1 cells by CRISPR/Cas 9 system. (b) CD97 knockout inhibited PPAR-γ protein level (A), while it increased p65 expression (B). (c) CD97 ectopic expression in CD97 knockout THP-1 cells increased PPAR-γ protein level (A) while it inhibited p65 expression (B). (d) TNF-α secretion was enhanced in CD97 knockout THP-1 cells while CD97 ectopic expression reversed this effect.</div>

Mediators of Inflammation

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Figure 5

Figure 5: CD97/ADGRE5 Inhibits LPS Induced NF-κB Activation through PPAR-γ Upregulation in Macrophages