A Single Bout of Fasting (24 h) Reduces Basal Cytokine Expression and Minimally Impacts the Sterile Inflammatory Response in the White Adipose Tissue of Normal Weight F344 Rats

Table 2

Effects of a 24 h fast on basal cytokine concentrations in WAT. Differences between WAT cytokines in fed (FED + 0IS, ) versus fasted (FASTED + 0IS, ) control rats by unpaired, 2-tailed -test. Values are expressed as means ± SEM.

	FED	FASTED	value	Percent change
Subcutaneous WAT
TNF-α (pg/mg)	4.32 ± 0.47	3.27 ± 0.19	0.06	−24.3%
IL-1β (pg/mg)	10.91 ± 0.7	6.72 ± 0.77	0.001	−38.5%
IL-6 (pg/mg)	2.15 ± 0.13	1.9 ± 0.03	0.05	−10.7%
IL-10 (pg/mg)	1.01 ± 0.06	0.75 ± 0.09	0.03	−26.7%
TNF-α/IL-10	4.4 ± 0.58	4.67 ± 0.42	0.72	6.1%
IL-1β/IL-10	10.99 ± 0.86	9.55 ± 1.15	0.34	−13.0%
Intraperitoneal WAT
TNF-α (pg/mg)	6.18 ± 0.35	6.85 ± 0.48	0.27	10.8%
IL-1β (pg/mg)	10.79 ± 1.28	10.46 ± 0.98	0.89	−2.1%
IL-6 (pg/mg)	25.65 ± 5.34	5.85 ± 1.04	0.003	−77.1%
IL-10 (pg/mg)	1.02 ± 0.07	1.02 ± 0.06	0.98	−0.2%
TNF-α/IL-10	6.09 ± 0.22	6.73 ± 0.21	0.05	10.3%
IL-1β/IL-10	10.57 ± 1.11	10.39 ± 0.71	0.89	−1.8%
Visceral WAT
TNF-α (pg/mg)	6.1 ± 0.46	4.05 ± 0.33	0.003	−33.6%
IL-1β (pg/mg)	9.78 ± 0.68	5.68 ± 0.74	0.001	−41.8%
IL-6 (pg/mg)	39.12 ± 5.24	15.66 ± 5.14	0.007	−59.9%
IL-10 (pg/mg)	1.2 ± 0.06	0.88 ± 0.10	0.02	−26.7%
TNF-α/IL-10	5.15 ± 0.43	4.45 ± 0.35	0.13	−17.5%
IL-1β/IL-10	8.18 ± 0.50	5.54 ± 0.52	0.003	−32.2%