CETP Lowers TLR4 Expression Which Attenuates the Inflammatory Response Induced by LPS and Polymicrobial Sepsis

Figure 4

Confocal microscopy of macrophages: colocalization between LPS and CETP (magnification of 600x). Representative confocal image of peritoneal macrophages from WT mice stimulated with LPS (1 µg/mL) for 4 h in the presence of the LBP, known to be attached to LPS: LBP was utilized as a positive control (a) or CETP (b) (1 µg/mL). FITC-LPS, green; nucleus, blue (DAPI); LBP, yellow; CD68⁺ macrophages, red; CETP, blue (in the absence of DAPI because CETP antibody is also blue); merge: colocalization between markers.

(a)

(b)