Inhibition of Macrophage Migration Inhibitory Factor Protects against Inflammation and Matrix Deposition in Kidney Tissues after Injury

Figure 1

Obstruction-induced matrix deposition and MIF expression in kidneys. (a) PAS and Masson’s trichrome staining of renal cortex sections revealed marked tubular dilation and atrophy associated with excessive matrix deposition and inflammatory cells infiltration. Bar: 50 μm. (b) Protein expression of type III collagen determined by immunohistochemical staining was upregulated in UUO kidneys compared with the sham. Bar: 100 μm. (c) qRT-PCR analysis indicated increased mRNA expressions of Col1α1 and Col3α1 in UUO kidneys. (d) ELISA assay showed enhanced TGF-β1 levels in UUO kidneys. (e) Western blot analysis showed increased MIF expression in UUO kidneys. (f) qRT-PCR revealed elevated mRNA expression of MIF in UUO kidneys. (g) Immunohistochemical staining identified upregulated expression of MIF and CD74 in UUO kidneys. Bar: 50 μm. (h) Enhanced CD68 expression reveals marked macrophage infiltration in UUO kidneys. Bar: 50 μm.

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