Mediators of Inflammation

Research Article

Central Interleukin-1β Suppresses the Nocturnal Secretion of Melatonin

Table 1

All genes analysed by Real-Time PCR are listed with their full names and abbreviations.


GenBank acc. number	Gene	Amplicon size (bp)	Forward/reverse	Sequence	Reference

NM_001034034	GAPDH glyceraldehyde-3-phosphate dehydrogenase	134	Forward	AGAAGGCTGGGGCTCACT	Herman et al. [65]
			Reverse	GGCATTGCTGACAATCTTGA

U39357	ACTB beta actin	168	Forward	CTTCCTTCCTGGGCATGG	Herman et al. [65]
			Reverse	GGGCAGTGATCTCTTTCTGC

BC108088.1	HDAC1 histone deacetylase 1	115	Forward	CTGGGGACCTACGGGATATT	Herman et al. [65]
			Reverse	GACATGACCGGCTTGAAAAT

XM_004016118	TPH1 tryptophan hydroxylase 1	128	Forward	CGTCCTGTGGCTGGTTACTT	Originally designed
			Reverse	TGGCAGGTATCTGGTTCTGG

NM_173907.2	DDC dopa decarboxylase	150	Forward	TTCTTGCTTCGTGGTGGCTA	Originally designed
			Reverse	CGGAACTCAGGGCAGATGAA

NM_001009461	AANAT aralkylamine N-acetyltransferase	154	Forward	CGAGAGGCCTTCATCTCTGT	Herman et al. [15]
			Reverse	GTCTCTCCTCATCCCACAGG

KC290950	HIOMT hydroxyindole O-methyltransferase	167	Forward	AGCTTCCATGAAGGGGATTT	Herman et al. [15]
			Reverse	AGGAGGCTCTCGATGACCAG

X54796.1	IL1B interleukin 1 beta	137	Forward	CAGCCGTGCAGTCAGTAAAA	Herman et al. [65]
			Reverse	GAAGCTCATGCAGAACACCA

NM_001206735.1	IL1R1 interleukin 1 receptor, type I	124	Forward	GGGAAGGGTCCACCTGTAAC	Herman et al. [65]
			Reverse	ACAATGCTTTCCCCAACGTA

NM_001046210.1	IL1R2 interleukin 1 receptor, type II	161	Forward	CGCCAGGCATACTCAGAAA	Originally designed
			Reverse	GAGAACGTGGCAGCTTCTTT

NM_001308595.1	IL1RN interleukin 1 receptor antagonist	145	Forward	AGGATCTGGGATGTCAACCA	Originally designed
			Reverse	CATGGATCCCCAGGAACATA

NM_001009392.1	IL6 interleukin 6	165	Forward	GTTCAATCAGGCGATTTGCT	Herman et al. [65]
			Reverse	CCTGCGATCTTTTCCTTCAG

NM_001110785	IL6R interleukin 6 receptor	149	Forward	TCAGCGACTCCGGAAACTAT	Herman et al. [65]
			Reverse	CCGAGGACTCCACTCACAAT

XM_004016974	IL6ST glycoprotein 130	139	Forward	GGCTTGCCTCCTGAAAAACC	Originally designed
			Reverse	ACTTCTCTGTTGCCCACTCAG

NM_001024860	TNF tumor necrosis factor	153	Forward	CAAATAACAAGCCGGTAGCC	Herman et al. [65]
			Reverse	AGATGAGGTAAAGCCCGTCA

NM_174674	TNFRSF1A tumor necrosis factor receptor, type 1	137	Forward	AGGTGCCGGGATGAAATGTT	Herman et al. [65]
			Reverse	CAGAGGCTGCAGTTCAGACA

NM_001040490	TNFRSF1B tumor necrosis factor receptor, type 2	122	Forward	ACCTTCTTCCTCCTCCCAAA	Herman et al. [65]
			Reverse	AGAAGCAGACCCAATGCTGT