Genetic Ablation of Soluble TNF Does Not Affect Lesion Size and Functional Recovery after Moderate Spinal Cord Injury in Mice

Figure 5

Flow cytometric analysis of the activation state of microglia and infiltrating cells 3 days after SCI. (a–c) Number of CD11b⁺ microglia (a) and CD11b⁺ cells (b) and percentages of microglia and infiltrating cells (c) within the lesion and in the perilesion area 3 days after SCI. (d) Representative histograms of MHCII expression on CD11b⁺ microglia and CD11b⁺ cells located in the lesions of and mice 3 days after SCI. (e, f) The number of MHCII⁺CD11b⁺ cells (e) and MFI for MHCII (f) were comparable between and mice. (g) Representative histograms of CD11c expression on microglia and infiltrating cells located in the lesions of and mice 3 days after SCI. (h, i) The number of CD11c⁺CD11b⁺ cells (h) and MFI for CD11c expression (i) were comparable between and mice. (j) Representative dot blots comparing CD11c expressions on MHCII⁺CD11b⁺ cells in and mice 3 days after SCI. (k) Percentages of MHCII⁺, CD11c⁺, and MHCII⁺CD11c⁺ cells in the lesion area. -5 mice/group. (l) Western blot analysis for Iba1 protein expression 3 days after SCI. Data are normalized to α-actin protein expression (/group). Results are presented as mean ± SD, , , Mann–Whitney and Wilcoxon matched-pairs signed rank tests.