Genetic Ablation of Soluble TNF Does Not Affect Lesion Size and Functional Recovery after Moderate Spinal Cord Injury in Mice

<div>Microglial and leukocyte immunoreactivity 35 days after SCI. (a–c) Immunohistochemical staining for F4/80 (a) and CD45 (b) and immunofluorescent staining for Iba1 (c) were comparable between <svg height="12.4254pt" id="M168" style="vertical-align:-0.1672001pt" version="1.1" viewbox="-0.0498162 -12.2582 57.5072 12.4254" width="57.5072pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M797 0V26C739 32 732 36 732 103V296C732 394 682 449 605 449C576 449 550 437 529 423C504 407 475 389 446 366C425 418 382 449 334 449C303 449 279 437 253 421C222 403 201 385 180 371V452C135 432 85 419 41 411V388C99 379 102 374 102 310V103C102 38 93 32 27 26V0H238V26C189 32 180 38 180 103V338C210 363 250 390 289 390C351 390 377 348 377 275V103C377 37 368 32 306 26V0H520V26C465 32 456 38 456 101V296C456 314 455 326 453 338C491 369 529 390 565 390C628 390 653 345 653 274V107C653 36 642 32 583 26V0H797Z" id="g121-107"></path><glyph.data ascent="989" descent="-360" horiz-adv-x="819" 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transform="matrix(.0091,0,0,-0.0091,40.8,-5.741)"><path d="M303 37L293 66C280 59 256 49 231 49S173 63 173 143V396H281C294 404 298 428 287 439H173V575L158 577L92 509V439H46L17 408L22 396H92V107C92 27 128 -12 192 -12C203 -12 218 -9 237 2L303 37Z" id="g58-114"></path><glyph.data ascent="1007" descent="-369" horiz-adv-x="312" vert-adv-y="312"></glyph.data></g><g transform="matrix(.0091,0,0,-0.0091,43.64,-5.741)"><path d="M381 704H317L48 -163H112L381 704Z" id="g50-48"></path><glyph.data ascent="3443" descent="-2856" horiz-adv-x="429" vert-adv-y="429"></glyph.data></g><g transform="matrix(.0091,0,0,-0.0091,47.547,-5.741)"><path d="M708 439H523V409C587 401 594 395 581 344C562 272 534 184 513 118H511C468 225 428 330 391 434H361C318 317 283 217 244 121H242C218 196 193 273 170 349C157 394 160 401 220 409V439H10V409C60 403 67 394 86 343C127 224 165 109 201 -10H233C273 101 314 199 353 299C393 195 432 99 475 -10H507C554 126 596 251 627 334C648 390 657 401 708 409V439Z" id="g58-117"></path><glyph.data ascent="1007" descent="-369" horiz-adv-x="698" vert-adv-y="698"></glyph.data></g><g transform="matrix(.0091,0,0,-0.0091,53.99,-5.741)"><path d="M303 37L293 66C280 59 256 49 231 49S173 63 173 143V396H281C294 404 298 428 287 439H173V575L158 577L92 509V439H46L17 408L22 396H92V107C92 27 128 -12 192 -12C203 -12 218 -9 237 2L303 37Z" id="g58-114"></path><glyph.data ascent="1007" descent="-369" horiz-adv-x="312" vert-adv-y="312"></glyph.data></g></svg> and mTNF<svg height="12.308pt" id="M169" style="vertical-align:-0.04979992pt" version="1.1" viewbox="-0.0498162 -12.2582 16.4636 12.308" width="16.4636pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M610 0V27L347 666L316 657L46 26V0H610ZM504 54H116L310 537L504 54Z" id="g50-133"></path><glyph.data ascent="3443" descent="-2856" horiz-adv-x="654" vert-adv-y="654"></glyph.data></g><g transform="matrix(.0091,0,0,-0.0091,5.947,-5.741)"><path d="M381 704H317L48 -163H112L381 704Z" id="g50-48"></path><glyph.data ascent="3443" descent="-2856" horiz-adv-x="429" vert-adv-y="429"></glyph.data></g><g transform="matrix(.0091,0,0,-0.0091,9.855,-5.741)"><path d="M610 0V27L347 666L316 657L46 26V0H610ZM504 54H116L310 537L504 54Z" id="g50-133"></path><glyph.data ascent="3443" descent="-2856" horiz-adv-x="654" vert-adv-y="654"></glyph.data></g></svg> mice 35 days after SCI. Analysis was based on 5 sections from each animal, <svg height="8.55521pt" id="M170" style="vertical-align:-0.2063904pt" version="1.1" viewbox="-0.0498162 -8.34882 27.8368 8.55521" width="27.8368pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M495 86L479 114C446 82 419 66 409 66C401 66 401 72 406 97C420 166 436 231 453 297C489 435 454 448 428 448C406 448 384 439 354 422C305 394 222 327 161 247H159L183 345C200 415 194 448 173 448C143 448 82 410 23 351L38 325C64 349 95 371 105 371C111 371 116 365 109 336L25 -4L31 -12C50 -4 77 3 107 9C119 69 132 122 145 168C197 254 321 381 370 381C387 381 393 374 378 305L329 95C309 17 320 -12 345 -12C372 -12 430 19 495 86Z" id="g113-111"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="502" vert-adv-y="502"></glyph.data></g><g transform="matrix(.013,0,0,-0.013,10.158,0)"><path d="M535 323V373H52V323H535ZM535 138V188H52V138H535Z" id="g117-34"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="587" vert-adv-y="587"></glyph.data></g><g transform="matrix(.013,0,0,-0.013,21.421,0)"><path d="M456 178V225H360V632H320C217 496 115 347 20 206V178H280V106C280 40 276 34 189 27V0H445V27C364 34 360 39 360 106V178H456ZM280 225H82C149 335 214 431 278 520H280V225Z" id="g113-53"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="480" vert-adv-y="480"></glyph.data></g></svg> mice/group. Scale bars: (a, b) low magnification = 200 <i>μ</i>m and high magnification = 100 <i>μ</i>m. (c) 40 <i>μ</i>m.</div>

Mediators of Inflammation

fig6

Figure 6

Figure 6: Genetic Ablation of Soluble TNF Does Not Affect Lesion Size and Functional Recovery after Moderate Spinal Cord Injury in Mice 