SOCS1 Mimetic Peptide Suppresses Chronic Intraocular Inflammatory Disease (Uveitis)

Figure 2

SOCS1-KIR conferred protection from posterior uveitis and pan uveitis. (a–d) We induced EAU in rats by adoptive transfer of uveitogenic R16 T cell line and the rats were treated with either SOCS1-KIR or scrambled peptide (control). The eyes were analyzed by histology on day 12 after disease induction. Representative results of histological analyses of the central retina (a), peripheral retina (b), iris and ciliary body (c), and the cornea (d) are shown. Images reveal substantial reduction of the numbers of inflammatory cells (arrows) in the vitreous and other tissues of rats treated with SOCS1-KIR compared to the scrambled peptide. Hallmark features of severe uveitis such as retinal folds and photoreceptor cell loss (asterisks), infiltrated inflammatory cells (arrows), and disorganized structure of retinae are markedly alleviated in eyes of rats treated with SOCS1-KIR. The sections were stained with hematoxylin and eosin. GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer; PRL, photoreceptor layer; OpN, optic nerve; CB, ciliary body; AC, anterior chamber. (e) Histological score and assessment of disease severity based on changes of the retina architecture, retinal vessels, and presence of retinal and choroidal infiltrates. Results represent at least three independent experiments. .

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