SOCS1 Mimetic Peptide Suppresses Chronic Intraocular Inflammatory Disease (Uveitis)

Figure 7

SOCS1-KIR has no effects on systemic immune response. We induced EAU by adoptive transfer of pathogenic R16 T cells and the rats were treated by topical administration of SOCS1-KIR or scrambled peptides as described above. Spleen cells from the rats were restimulated in vitro with indicated peptide and analyzed by the thymidine incorporation (a) or intracellular cytokine staining assay (b, c). Proliferative responses were analyzed in six replicate cultures and data presented as CPM (a). Numbers in quadrants indicate percentage of CD4⁺ T cells expressing IL-17 or IFN-γ (b, c). = n.s. (No significance) > 0.05 (Student’s two-tailed -test).

(a)

(b)

(c)