Doxycycline Inhibits IL-17-Stimulated MMP-9 Expression by Downregulating ERK1/2 Activation: Implications in Myogenic Differentiation

Figure 2

MMP-9 is highly induced by IL-17 during myogenic differentiation of C2C12 cells. (a) C2C12 cells were cultured in myogenic differentiation medium (MDM) with or without 50 ng/mL of IL-17 and compared to cells cultured in GM with or without IL-17. MMP-9 activity in conditioned media was determined by zymography. MMP-9 mRNA transcript production in C2C12 cells was determined by RT-PCR analysis. GAPDH was used as a gel loading control. Quantification plot is presented above the zymography. (b) MCK and MyHC expression in C2C12 cells during myogenic differentiation with or without 50 ng/mL of IL-17 determined by RT-PCR and Western blot analysis, respectively. α-tubulin and GAPDH were used as gel loading controls.

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