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Mediators of Inflammation
Volume 2016, Article ID 3583684, 11 pages
http://dx.doi.org/10.1155/2016/3583684
Research Article

In Vitro Anti-Inflammatory Effects of Three Fatty Acids from Royal Jelly

1College of Animal Sciences, Zhejiang University, Hangzhou 310058, China
2Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China

Received 8 August 2016; Accepted 18 September 2016

Academic Editor: Jie Yin

Copyright © 2016 Yi-Fan Chen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Trans-10-hydroxy-2-decenoic acid (10-H2DA), 10-hydroxydecanoic acid (10-HDAA), and sebacic acid (SEA) are the three major fatty acids in royal jelly (RJ). Previous studies have revealed several pharmacological activities of 10-H2DA and 10-HDAA, although the anti-inflammatory effects and underlying mechanisms by which SEA acts are poorly understood. In the present study, we evaluated and compared the in vitro anti-inflammatory effects of these RJ fatty acids in lipopolysaccharide-stimulated RAW 264.7 macrophages. The results showed that 10-H2DA, 10-HDAA, and SEA had potent, dose-dependent inhibitory effects on the release of the major inflammatory-mediators, nitric oxide, and interleukin-10, and only SEA decreased TNF-α production. Several key inflammatory genes have also been modulated by these RJ fatty acids, with 10-H2DA showing distinct modulating effects as compared to the other two FAs. Furthermore, we found that these three FAs regulated several proteins involved in MAPK and NF-κB signaling pathways. Taken together, these findings provide additional references for using RJ against inflammatory diseases.