Matrine Attenuates COX-2 and ICAM-1 Expressions in Human Lung Epithelial Cells and Prevents Acute Lung Injury in LPS-Induced Mice

Figure 3

Matrine inhibits the nuclear translocation of NF-κB in A549 cells. (a) Effects of matrine on the LPS-induced phosphorylation of IκB-α, with total IκB-α levels used as internal controls. (b) To assess the nuclear translocation of NF-κB, cells were pretreated with different matrine concentrations for 1 h and then incubated with LPS (1 μg/mL) for 1 h. The internal controls were lamin B1 in the nucleus and β-actin in the cytosol. (c) Matrine suppressed luciferase activity by the NF-κB promoter assay. Three independent experiments were analyzed and compared with the LPS-treated control group. Data are presented as the mean ± SEM. compared to LPS alone.

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