Matrine Attenuates COX-2 and ICAM-1 Expressions in Human Lung Epithelial Cells and Prevents Acute Lung Injury in LPS-Induced Mice

Table 1

Primers used in real-time PCR analyses of mRNA expression.

Gene	Primers	(5′-3′ sequence)	GenBank accession number	Product size (bp)
IL-1β	Forward; Reverse	CACTACAGGCTCCGAGATGA; CGTTGCTTGGTTCTCCTTGT	NM_008361	143
IL-4	Forward; Reverse	TCCGTGCTTGAAGAAGAACTC; GTGATGTGGACTTGGACTCATT	NM_021283.2	116
IL-6	Forward; Reverse	AGGACCAAGACCATCCAATTCA; GCTTAGGCATAACGCACTAGG	NM_031168.1	97
IL-13	Forward; Reverse	GCTCCAGCATTGAAGCAGTG; CGTGGCAGACAGGAGTGTT	NM_008355.3	141
TNF-α	Forward; Reverse	GCACCACCATCAAGGACTC; AGGCAACCTGACCACTCTC	NM_013693	96
MCP-1	Forward; Reverse	TTCCACAACCACCTCAAGCA; TTAAGGCATCACAGTCCGAGTC	NM_011333	80
CCL5	Forward; Reverse	CGAAGGAACCGCCAAGTGT; AGGACTAGAGCAAGCAATGAC	NM_013653.3	139
CCL11	Forward; Reverse	GGCTTCATGTAGTTCCAGAT; CCATTGTGTTCCTCAATAATCC	NM_011330.3	145
CCL24	Forward; Reverse	AGGCAGTGAGAACCAAGT; GCGTCAATACCTATGTCCAA	NM_019577.4	102
iNOS	Forward; Reverse	TTCCACAACCACCTCAAGCA; TTAAGGCATCACAGTCCGAGTC	NM_010927	83
COX-2	Forward; Reverse	ACCAGCAGTTCCAGTATCAGA; CAGGAGGATGGAGTTGTTGTAG	NM_011198	143
ICAM-1	Forward; Reverse	AACAGAATGGTAGACAGCAT; TCCACCGAGTCCTCTTAG	NM_010493.2	113
β-actin	Forward; Reverse	AAGACCTCTATGCCAACACAGT; AGCCAGAGCAGTAATCTCCTTC	NM_007393.3	92