Osteoclasts Are Required for Hematopoietic Stem and Progenitor Cell Mobilization but Not for Stress Erythropoiesis in Plasmodium chabaudi adami Murine Malaria

Figure 3

Modulation of splenic hematopoiesis during Plasmodium infection by calcitonin. Mice were treated with calcitonin (white bars) or with PBS (grey bars) during 5 consecutive days and were infected with P. c. adami DK parasites (10⁵ pRBCs) on the 3rd day of treatment. Data from uninfected controls (Ctl, Cal ) is included for comparison. Infected mice were sacrificed at 5 (), 8 (–12), and 10 (-4) days after infection and the spleen was aseptically recovered for analysis of numbers of total cells (a), erythroid cells (b), lineage⁻ cells (c), and hematopoietic stem cells (e) by cytofluorometry. Identification panel of hematopoietic stem cells (Lineage-7AAD⁻Sca1⁺cKit⁺) by cytofluorometry is also represented (d). Data are mean ± SEM and values are compared to respective uninfected control mice and between the two infected groups using a one-way ANOVA test. ; ; .