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Mediators of Inflammation
Volume 2016, Article ID 4374375, 12 pages
Research Article

Chitosan as an Immunomodulating Adjuvant on T-Cells and Antigen-Presenting Cells in Herpes Simplex Virus Type 1 Infection

1Department of Microbiology, Ajou University School of Medicine, Suwon, Republic of Korea
2Department of Applied Chemistry and Biological Engineering, Ajou University, Suwon, Republic of Korea
3Department of Biomedical Sciences, Ajou University, Suwon, Republic of Korea

Received 20 July 2016; Accepted 12 October 2016

Academic Editor: Teresa Zelante

Copyright © 2016 Bunsoon Choi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Herpes disease caused by herpes simplex virus type 1 (HSV-1) is an intractable condition. It is a major concern in public health. Our purpose of this study was to verify the function of chitosan as an adjuvant for immune regulation specifically under herpes simplex virus type 1 (HSV-1) infection. Ahead of HSV infection, chitosan, heat inactivated green fluorescent protein expressing HSV (G-HSV), and a combination of chitosan and G-HSV were used to pretreat ICR mice followed by HSV-1 infection. Using flow cytometric analysis, the frequencies of T-cells, monocytes, dendritic cells (DCs), and natural killer (NK) cells were analyzed by surface expression of , , , , , and cells. In HSV infected mice, chitosan treatment significantly increased the frequencies of T-cells (%) compared to those in the control group (%, ). The frequencies of DC and NK cells were also significantly different between chitosan treated mice and control mice. In addition, anti-HSV IgG antibody was downregulated in chitosan treated mice. These results suggest that chitosan is a potential modulator or immune stimulator as an adjuvant in HSV-1 infected mice.