Chitosan as an Immunomodulating Adjuvant on T-Cells and Antigen-Presenting Cells in Herpes Simplex Virus Type 1 Infection

Figure 1

Efficacy of chitosan on and T-cells with or without HSV infection. Administration of 1% acetic acid (control), 0.23% chitosan (Chit), heat inactivated GFP-HSV (G-HSV), or chitosan with G-HSV (Chit+G-HSV) orally into normal mice for three times at 10-day intervals. On the next day, mice were sacrificed or inoculated with HSV-1 at 10 days later as described in the Materials and Methods. Isolated PBMC and lymph node cells were applied for analysis of and T-cells by flow cytometry (a–d). The frequencies of and T-cells in PBMC were significantly upregulated in chitosan treated mice compared to those in control mice infected with HSV. (e) Representative histogram of the frequencies of and cells in HSV infected PBMC. The number of mice used in each group was five. In (c) and and in (d) .

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