Kinin Peptides Enhance Inflammatory and Oxidative Responses Promoting Apoptosis in a Parkinson’s Disease Cellular Model
The effect of MPP+ on B1R expression in differentiated SK-N-SH cells. Confluent cells were incubated with MPP+ at different time intervals. (a) The changes of B1R mRNA expression after incubation at different time intervals were analyzed by Real-Time PCR and are presented as relative mean values in comparison to untreated cells, assumed to possess value equal to 1 (dashed line). The expression of B1R protein was analyzed by immunocytochemistry with specific antibodies and the quantification of fluorescence signal was performed as described in Materials and Methods (b). The results are shown as mean ± SD of the changes in comparison to untreated cells, assumed to possess 100% expression (dashed line). (c) The photographs show B1R expression on differentiated SK-N-SH cells without or after 5-hour incubation with MPP+. versus untreated cells.