Research Article

Kinin Peptides Enhance Inflammatory and Oxidative Responses Promoting Apoptosis in a Parkinson’s Disease Cellular Model

Figure 7

The effect of MPP+ on B2R expression in differentiated SK-N-SH cells. Confluent cells were incubated with MPP+ at different time intervals. (a) Real-Time PCR analysis of the B2R mRNA expression after different incubation time. mRNA expression is shown as relative mean values as compared to untreated cells, assumed to possess value equal to 1 (dashed line). The expression of B2R protein was analyzed by immunocytochemistry with specific antibodies and the quantification of fluorescence signal was performed as described in Materials and Methods (b). The results are shown as mean ± SD of the changes in comparison to untreated cells, assumed to possess 100% expression (dashed line). (c) The photographs show B2R expression by differentiated SK-N-SH cells without or after 5-hour incubation with MPP+. versus untreated cells.