Kinin Peptides Enhance Inflammatory and Oxidative Responses Promoting Apoptosis in a Parkinson’s Disease Cellular Model
The effect of kinin peptides on D2R expression in differentiated SK-N-SH cells. The mRNA expression of D2R was analyzed with Real-Time PCR after BK (a) or DAKD (b) treatment of confluent cells at different time intervals. D2R mRNA expression is shown as relative mean values as compared to untreated cells, assumed to possess value equal to 1 (dashed line). The expression of D2R protein was analyzed by immunocytochemistry with specific antibodies and the quantification of fluorescence signal was performed as described in Materials and Methods (c, d). The results are shown as mean ± SD of the changes in comparison to untreated cells, assumed to possess 100% expression (dashed line). The photographs show D2R expression on differentiated SK-N-SH cells without or after 3-hour incubation with BK or DAKD (e). versus untreated cells.