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Mediators of Inflammation
Volume 2016 (2016), Article ID 4706915, 16 pages
Research Article

Inhibition of TLR4 Signalling-Induced Inflammation Attenuates Secondary Injury after Diffuse Axonal Injury in Rats

1Department of Neurosurgery, The First Affiliated Hospital of Xi’an Jiaotong University, 277 West Yanta Road, Xi’an, Shaanxi 710061, China
2Department of Neurosurgery, The Second Affiliated Hospital of Xi’an Jiaotong University, No. 157 of Xiwu Road, Xi’an, Shaanxi 710004, China

Received 19 April 2016; Revised 17 June 2016; Accepted 19 June 2016

Academic Editor: Ulrich Eisel

Copyright © 2016 Yonglin Zhao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

SD rats were randomly divided into control, DAI 12 h, DAI 1 d and DAI 3 d groups according to different time points after DAI. The expression of proteins related to the TLR4/NF-βB signalling pathway was detected in these groups and peaked at 1d post DAI. Then, TLR4 was found localized in neuron and microglia in DAI 1 d group. TLR4 inhibitor, TAK-242 was intravenously injected to DAI 1 d group at doses of 0.5 mg/kg next. Compared to DAI 1 d and DAI 1 d+vehicle groups, DAI 1 d+TAK-242 group showed decreased cell apoptosis, pathological changes, neuronal and axonal injury and glial reaction. Finally, the TLR4/NF-βB signalling pathway was involved in the neuroprotective effect induced by TAK-242.

  1. Supplementary Material