Cannabinoid CB2 Receptor Mediates Nicotine-Induced Anti-Inflammation in N9 Microglial Cells Exposed to β Amyloid via Protein Kinase C

Figure 3

CB2 receptor antagonist reversed nicotine-induced effects on iNOS and Arg-1 protein expressions. The microglial cells were divided into five groups: control: cells cultured in drug-free medium; Aβ: cells cultured in the medium containing 5 μM Aβ; nicotine (Nico) + Aβ: cells cultured in the medium containing 10 μM nicotine and 5 μM Aβ; AM630 + Nico + Aβ: cells cultured in the medium containing 10 μM CB2 antagonist AM630, 10 μM nicotine, and 5 μM Aβ; AM630 + Aβ: cells cultured in the medium containing 10 μM AM630 and 5 μM Aβ. After an incubation of 24 h, western blot () and immunocytochemistry were used to evaluate iNOS (a-b) and Arg-1 (c-d) expressions. Results are expressed as means ± SD, ; n.s.: no significance. Bar = 10 μm.

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