Research Article

Cannabinoid CB2 Receptor Mediates Nicotine-Induced Anti-Inflammation in N9 Microglial Cells Exposed to β Amyloid via Protein Kinase C

Figure 5

CB2 receptor antagonist abolished nicotine-induced effect on PKC expression. The microglial cells were divided into five groups: control: cells cultured in drug-free medium; Aβ: cells cultured in the medium containing 5 μM Aβ; nicotine (Nico) + Aβ: cells cultured in the medium containing 10 μM nicotine and 5 μM Aβ; AM630 + Nico + Aβ: cells cultured in the medium containing 10 μM CB2 antagonist AM630, 10 μM nicotine, and 5 μM Aβ; AM630 + Aβ: cells cultured in the medium containing 10 μM AM630 and 5 μM Aβ. After an incubation of 24 h, western blot was used to detect PKC expression (). Results are expressed as means ± SD, ; n.s.: no significance.