Folic Acid Is Able to Polarize the Inflammatory Response in LPS Activated Microglia by Regulating Multiple Signaling Pathways

Figure 6

Modulation of SOCS proteins in BV-2 treated with folic acid. (a) Cells were treated with the indicated doses of folic acid (5, 10, 30, and 50 μg/mL) for 1 h before LPS treatment. Total cell lysates were prepared for western blot analysis for the content of SOCS1 and SOCS3 in BV-2 cells. Protein levels were determined by measuring immunoblot band intensities by scanning densitometry. (b) mRNA expression determined by real-time RT-PCR. BV-2 microglial cells were incubated for 6 h with LPS alone or after 1 h of pretreatment with different amounts of folates. Data represent the means ± S.D. of five separate experiments. .

(a)

(b)