Pharmacological Inactivation of Src Family Kinases Inhibits LPS-Induced TNF-α Production in PBMC of Patients with Behçet’s Disease

Figure 2

The effect of the PP2 on LPS-induced cytokine/chemokine production in PBMC. 0.5 × 10⁶ PBMC were treated with 10 μM PP2 for 1 h prior to stimulation with 100 ng/mL LPS for 18 h. Supernatants were collected and cytokine/chemokine levels determined by ELISA; TNF-α (a), IL-6 (b), and IL-8 (c). The results are presented as pooled data from three independent experiments. The statistical significance of the data was analyzed by Student’s -test.

(a)

(b)

(c)