Pharmacological Inactivation of Src Family Kinases Inhibits LPS-Induced TNF-α Production in PBMC of Patients with Behçet’s Disease

Figure 3

Inhibition of LPS-induced TNF-α production by PP2 in PBMC of active BD patients. Five × 10⁶ PBMC were treated with 10 μM PP2 for 1 h before 100 ng/mL LPS stimulation for 18 h. Cell lysates were examined for the presence of TNF-α by Western Blotting. The lowest panel represents loading control (β-actin). The image shown represents a single representative example of four separate experiments.