Peroxisome Proliferator-Activated Receptor α Reduces Endothelin-1-Caused Cardiomyocyte Hypertrophy by Inhibiting Nuclear Factor-κB and Adiponectin

Figure 2

ETA receptor involved in ET-1-dependent hypertrophy and adiponectin expression. The role of ET-1 receptor and ET-1 in myocardial cell size was determined in cells pretreated with ETA receptor antagonist (BQ123) or ETB receptor antagonist (BQ788) for 1 h, followed by stimulation with or without ET-1 (50 nM) for 48 h. Cells were fixed and stained with rhodamine-phalloidin (red) and DAPI (blue) followed by cell surface area quantitation (scale bar, 50 μm) (a). Data are expressed as mean ± SEM of values from three independent experiments (b). ³H-leucine incorporation was determined as a measure of relative protein synthesis (c). Immunoblot assay and quantification of adiponectin (AND), beta-myosin heavy chain (β-MHC), and B-type natriuretic peptide (BNP) expression were also demonstrated (d). , compared with untreated control group.

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