Adiponectin Inhibits LPS-Induced HMGB1 Release through an AMP Kinase and Heme Oxygenase-1-Dependent Pathway in RAW 264 Macrophage Cells

Figure 6

Effect of kinase inhibitors on the suppression by adiponectin of LPS-induced HMGB1 release. Raw 264 cells were cultured as described in Figure 1 legend and were treated with compound C (a) or SB203580 (b) for 1 h before treatment with adiponectin (10 μg/mL) for 18 h, following which they were stimulated with LPS (200 ng/mL) for another 24 h. Culture media were collected and analyzed by HMGB1 western blotting followed by quantification of the intensity of the chemiluminescent HMGB1 band. The results are expressed as means ± SE of three independent experiments ( and significance compared with control; significance compared with LPS treated cells; significance compared with LPS plus SB203580 treated cells).

(a)

(b)