Indoxyl Sulfate Induces Mesangial Cell Proliferation via the Induction of COX-2

Figure 4

Expression of COX-2 in IS-induced mouse MCs. MCs were treated with the indicated doses of IS (0, 250, and 500 μM) for 24 h and then COX-2 protein (a and b) and mRNA (c) and protein expressions were analyzed by Western blotting and qRT-PCR. A time course analysis of COX-2 mRNA expression following IS treatment at a dose of 500 μM was also examined (d). Values are means ± SD; in each group; versus control and versus control.

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(b)

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(d)