Anti-Inflammatory and Antimicrobial Effects of Estradiol in Bovine Mammary Epithelial Cells during Staphylococcus aureus Internalization

Table 1

Oligonucleotides used in this study.

Gene (bovine)	Primer	Sequence (5′ → 3′)	Fragment size (bp)	Tm (°C)	References
TAP	F	GCGCTCCTCTTCCTGGTCCTG	216	57	[14]
	R	GCACGTTCTGACTGGGCATTGA
LAP	F	GCCAGCATGAGGCTCCATC	194	54	[14]
	R	CTCCTGCAGCATTTTACTTGGGCT
DEFB1	F	CCATCACCTGCTCCTCACA	185	54	[14]
	R	ACCTCCACCTGCAGCATT
BNDB4	F	GCCAGCATGAGGCTCCATC	278	54	This study
	R	CGTTTAAATTTAGACGGTGT
BNBD5	F	GCCAGCTGAGGCTCCATC	143	55	[14]
	R	TTGCCAGGGCACGAGATCG
BNBD10	F	GCTCCATCACCTGCTCCTC	152	54	[14]
	R	AGGTGCCAATCTGTCTCATGA
GAPDH	F	TCAACGGGAAGCTCACTGG	237	57	[14]
	R	CCCCAGCATCGAAGGTAGA
α5 integrin	F	TGCAGTGTGAGGCCGTGTATGAAG	230	58	[10]
	R	CGGGAGGGAGCGTTTGAAGAAT
β1 integrin	F	TGCGAGTGTGGTTGCCTGTAAGT	123	60	[10]
	R	ATTGAAGGCTCGGCACTGAACA
TLR2	F	CGACTGGCCCGATGACTACC	146	58.5	[15]
	R	TGAGCAGGAGCAACAGGAAGAG
TNF-α	F	CCCCTGGAGATAACCTCCCA	101	56	[15]
	R	CAGACGGGAGACAGGAGAGC
IL-1β	F	GCAGAAGGGAAGGGAAGAATGTAG	198	52	[15]
	R	CAGGCTGGCTTTGAGTGAGTAGAA
IL-6	F	AACCACTCCAGCCACAAACACT	179	57	[15]
	R	GAATGCCCAGGAACTACCACAA
IL-8	F	TTCCACACCTTTCCACCCCAA	149	53.5	[15]
	R	GCACAACCTTCTGCACCCACTT
IL-10	F	GATGCGAGCACCCTGTCTGA	129	59	[15]
	R	GCTGTGCAGTTGGTCCTTCATT
S100A7 (psoriasin)	F	GCAGCTCTCAGCTTGAGCAG	221	54	[14]
	R	CCAGCAAGGACAGGAACTCAG
ERα	F	TTCGGAAGTGCTATGAGGTTG	139	55	This study
	R	GATATTCTTTGTGTTGGAGTTTTA
ERβ	F	GACCCTACCAGACCTTTCAGTG	153	60	This study
	R	TGCTCCATGCCTTTGTTGC

TAP: Tracheal Antimicrobial Peptide; LAP: Lingual Antimicrobial Peptide; DEFB1: Defensin Beta 1; BNBD4: Bovine Neutrophil Beta Defensin 4; BNBD5: Bovine Neutrophil Beta Defensin 5; BNDB10: Bovine Neutrophil Beta Defensin 10; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase; TLR2: Toll-Like Receptor 2; TNF-α: Tumor Necrosis Factor-α; IL-1β: Interleukin 1-β; IL-6: Interleukin-6; IL-8: Interleukin-8; IL-10: Interleukin-10; S100A7: S100 Calcium-binding protein A7; ERα: Estrogen Receptor α; ERβ: Estrogen Receptor β.