C-Terminal Alpha-1 Antitrypsin Peptide: A New Sepsis Biomarker with Immunomodulatory Function

Figure 3

Neutrophil viability in response to CAAP48, CAAP47, scrambled peptide, and VIRIP. After 30 min peptide incubation, PMN (1 × 10⁶ cells/mL) were double stained with Annexin V-FITC and propidium iodide (PI) for 15 min and analyzed by flow cytometry. The percentage of viable cells (PI and Annexin negative) is illustrated for different peptide concentrations. CAAP48 (a) leads to a marked reduction of the viability of neutrophils already at 5 μM (24 ng/μL) while CAAP47 (b) influences viability of PMN not before 40 μM (192 ng/μL). Both the scrambled peptide (c) and VIRIP (d) had no effect. Data of four independent experiments are shown. PMA was used as positive control and 1x PBS diluted in RPMI 1640 (1 : 2) as well as PMN in RPMI 1640 without PBS served as negative control.

(a)

(b)

(c)

(d)