Research Article

Th17 Cell Response in Mice following Motor Nerve Injury

Figure 1

Inflammation and CD4 T cell activation in mice. (a) Cervical lymph nodes were collected from uninjured (Con) and axotomized WT and mice at 7, 9, and 14 dpa. The average of total number of dCLN from one mouse was calculated and compared ( mice/group). (b) dCLN from 3 individual mice in each group were collected and photographed on 7 dpa (the underline denotes the horizontal dimension of each dCLN). (c) dCLN were gated on T cells (CD3+ cells) and further analyzed for their subsets: CD4+ and CD8+ T cells. (d) the average of CD4+ and CD8+ T cell percentages in each group was calculated and compared. (e) The total numbers of CD4+ and CD8+ T cells were calculated on the basis of the total number of corresponding dCLN and their percentages. (f) dCLN cells were gated on CD4+ T cells and analyzed for CD69+ cells (in the square gate). (g) the average of CD69+ cell percentages in each group was calculated and compared. (h) The total number of CD69+ cells was calculated on the basis of the total number of corresponding dCLN and their percentages. (i) dCLN cells were gated on CD4+ T cells and analyzed for cells (in the square gate). (j) the average of cell percentages in each group was calculated and compared. (k) The total number of cells was calculated on the basis of the total number of corresponding dCLN and their percentages (data are presented as mean ± SD; mice/group, ; blue bars represent uninjured animals, whereas gold bars represent animals that have been subjected to axotomy).
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